Journal:

Article Title: Human and avian influenza viruses target different cell types in cultures of human airway epithelium

doi: 10.1073/pnas.0308001101

Figure Lengend Snippet: Cellular tropism of human and avian influenza viruses in differentiated cultures of nasal epithelial cells. The cultures were infected with either Mem96-M (a) or DkH1 (b) viruses, fixed 7 h after infection, and immunostained for cilia (gray) and virus antigen (red). (Bars = 10 μm.)

Article Snippet: Primary human epithelial cells from tracheal/bronchial and nasal tissues were purchased from Clonetics and Promo Cell, Heidelberg, respectively.

Techniques: Infection

Journal: Translational Vision Science & Technology

Article Title: Differential Effect of Proinflammatory Cytokines on Corneal and Conjunctival Epithelial Cell Mucins and Glycocalyx

doi: 10.1167/tvst.10.7.17

Figure Lengend Snippet: Effect of IL-6 on MUC1, MUC4, MUC16 gene, and protein expression in stratified cultures of human corneal ( A, B ) and conjunctival ( C, D ) epithelial cells. The cells were exposed to IL-6 for 24 hours. * P < 0.05 compared to control cells ( C ) that were not exposed to IL-6.

Article Snippet: Human corneal epithelial cells were propagated in keratinocyte growth medium supplemented with bovine pituitary extract (0.004 mL/mL), human epidermal growth factor (0.125 ng/mL), human insulin (5 µg/mL), hydrocortisone (0.33 µg/mL), epinephrine (0.39 µg/mL), transferrin (10 µg/mL), and calcium chloride (0.15 mM; PromoCell GmbH, Heidelberg, Germany).

Techniques: Expressing

Journal: Translational Vision Science & Technology

Article Title: Differential Effect of Proinflammatory Cytokines on Corneal and Conjunctival Epithelial Cell Mucins and Glycocalyx

doi: 10.1167/tvst.10.7.17

Figure Lengend Snippet: Effect of TNF-α on MUC1, MUC4, MUC16 gene, and protein expression in stratified cultures of human corneal ( A, B ) and conjunctival ( C, D ) epithelial cells. The cells were exposed to TNF-α for 24 hours. * P < 0.05 compared to control cells ( C ) that were not exposed to TNF-α.

Article Snippet: Human corneal epithelial cells were propagated in keratinocyte growth medium supplemented with bovine pituitary extract (0.004 mL/mL), human epidermal growth factor (0.125 ng/mL), human insulin (5 µg/mL), hydrocortisone (0.33 µg/mL), epinephrine (0.39 µg/mL), transferrin (10 µg/mL), and calcium chloride (0.15 mM; PromoCell GmbH, Heidelberg, Germany).

Techniques: Expressing

Journal: Translational Vision Science & Technology

Article Title: Differential Effect of Proinflammatory Cytokines on Corneal and Conjunctival Epithelial Cell Mucins and Glycocalyx

doi: 10.1167/tvst.10.7.17

Figure Lengend Snippet: Effect of IFN-γ on MUC1, MUC4, and MUC16 gene expression in stratified cultures of human corneal ( A, B ) and conjunctival ( C, D ) epithelial cells. The cells were exposed to IFN-γ for 24 hours. * P < 0.05 compared to control cells ( C ) that were not exposed to IFN-γ.

Article Snippet: Human corneal epithelial cells were propagated in keratinocyte growth medium supplemented with bovine pituitary extract (0.004 mL/mL), human epidermal growth factor (0.125 ng/mL), human insulin (5 µg/mL), hydrocortisone (0.33 µg/mL), epinephrine (0.39 µg/mL), transferrin (10 µg/mL), and calcium chloride (0.15 mM; PromoCell GmbH, Heidelberg, Germany).

Techniques: Expressing

Journal: Translational Vision Science & Technology

Article Title: Differential Effect of Proinflammatory Cytokines on Corneal and Conjunctival Epithelial Cell Mucins and Glycocalyx

doi: 10.1167/tvst.10.7.17

Figure Lengend Snippet: Representative confocal Z-stack images showing glycocalyx staining in stratified cultures of human corneal epithelial cells exposed to IL-6, TNF-α, and IFN-γ (250 pg/mL) for 24 hours. Nuclei are stained blue with DAPI and glycocalyx is stained green with Alexa-488 conjugated wheat germ agglutinin lectin. Scale bar = 100 µm.

Article Snippet: Human corneal epithelial cells were propagated in keratinocyte growth medium supplemented with bovine pituitary extract (0.004 mL/mL), human epidermal growth factor (0.125 ng/mL), human insulin (5 µg/mL), hydrocortisone (0.33 µg/mL), epinephrine (0.39 µg/mL), transferrin (10 µg/mL), and calcium chloride (0.15 mM; PromoCell GmbH, Heidelberg, Germany).

Techniques: Staining

Journal: Translational Vision Science & Technology

Article Title: Differential Effect of Proinflammatory Cytokines on Corneal and Conjunctival Epithelial Cell Mucins and Glycocalyx

doi: 10.1167/tvst.10.7.17

Figure Lengend Snippet: Representative confocal Z-stack images showing glycocalyx staining in stratified cultures of human conjunctival epithelial cells exposed to IL-6, TNF-α, and IFN-γ (250 pg/mL) for 24 hours. Nuclei are stained blue with DAPI and glycocalyx is stained green with Alexa-488 conjugated wheat germ agglutinin lectin. Scale bar = 100 µm.

Article Snippet: Human corneal epithelial cells were propagated in keratinocyte growth medium supplemented with bovine pituitary extract (0.004 mL/mL), human epidermal growth factor (0.125 ng/mL), human insulin (5 µg/mL), hydrocortisone (0.33 µg/mL), epinephrine (0.39 µg/mL), transferrin (10 µg/mL), and calcium chloride (0.15 mM; PromoCell GmbH, Heidelberg, Germany).

Techniques: Staining

Journal: Translational Vision Science & Technology

Article Title: Differential Effect of Proinflammatory Cytokines on Corneal and Conjunctival Epithelial Cell Mucins and Glycocalyx

doi: 10.1167/tvst.10.7.17

Figure Lengend Snippet: Flow cytometry quantification of apoptosis and necrosis in stratified human corneal ( A ) and conjunctival ( B ) epithelial cells treatment with IL-6, TNF-α, and IFN-γ (250 pg/mL) for 24 hours. The cells were stained with annexin V/propidium iodide dual staining for flow cytometry quantification. * P < 0.05 as compared to control cells that were not exposed to any cytokine.

Article Snippet: Human corneal epithelial cells were propagated in keratinocyte growth medium supplemented with bovine pituitary extract (0.004 mL/mL), human epidermal growth factor (0.125 ng/mL), human insulin (5 µg/mL), hydrocortisone (0.33 µg/mL), epinephrine (0.39 µg/mL), transferrin (10 µg/mL), and calcium chloride (0.15 mM; PromoCell GmbH, Heidelberg, Germany).

Techniques: Flow Cytometry, Staining

Journal: Translational Vision Science & Technology

Article Title: Differential Effect of Proinflammatory Cytokines on Corneal and Conjunctival Epithelial Cell Mucins and Glycocalyx

doi: 10.1167/tvst.10.7.17

Figure Lengend Snippet: Representative phase contrast microscopy images of stratified human corneal ( A ) and conjunctival ( B ) epithelial cells exposed to IL-6, TNF-α, and IFN-γ (250 pg/mL) for 24 hours. Scale bar = 100 µm.

Article Snippet: Human corneal epithelial cells were propagated in keratinocyte growth medium supplemented with bovine pituitary extract (0.004 mL/mL), human epidermal growth factor (0.125 ng/mL), human insulin (5 µg/mL), hydrocortisone (0.33 µg/mL), epinephrine (0.39 µg/mL), transferrin (10 µg/mL), and calcium chloride (0.15 mM; PromoCell GmbH, Heidelberg, Germany).

Techniques: Microscopy

Journal: Cell Research

Article Title: Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis

doi: 10.1038/cr.2011.148

Figure Lengend Snippet: List of cultured cells used in the study

Article Snippet: HREpic , NA , Human renal epithelial cells, passage 12; Sciencell .

Techniques: Cell Culture